Application of freeze-drying machine in preparation of calcium alginate sponge medical dressing

Calcium alginate sponge medical dressing is formed by freeze-drying machine vacuum low-temperature freeze-drying, calcium ion cross-linking curing and softener treatment

  The method for preparing a calcium alginate sponge medical dressing by means of a lyophilizer comprises the following steps:

  1 Preparation of sodium alginate blend solution According to the mass of distilled water 4. 2% 5. 4% weighed sodium alginate, and added it to distilled water to stir and dissolve uniformly to obtain a solution of low molecular weight sodium alginate. Then, high molecular weight sodium alginate was weighed according to the mass of distilled water of 0.6% 1.8%, added to the above solution, and stirred at room temperature until dissolved uniformly to obtain a sodium alginate blend solution.

  2 Preparation of sodium alginate sponge The sodium alginate blending solution prepared in step A is placed in a cryostat, and the solution is cooled while mechanically stirring. The solution is cooled to 0 ° C and 5 ° C to stop stirring and dispensed into the mold. The mold was placed in Shanghai Tuo-86 degree ultra-low temperature freezer for pre-freezing. The pre-freezing temperature was -80 °C -10 °C, and the pre-freezing time was 15 h. After the pre-freezing, the mold was placed in the Shanghai Tuoba Freeze Dryer TF- In SFD-5, the drying temperature was -50 ° C - 20 ° C, the degree of vacuum was 20 or less, and the treatment time was 6 36 h. After drying, the mold was released to obtain a shaped sodium alginate sponge.

  3 Preparation of calcium alginate sponge According to 2% 5% of the mass of ethanol, weigh the softener, add the weighed softener to the ethanol, stir until it is evenly mixed, and obtain a mixed solution of ethanol and softener, and then according to the quality of ethanol. 3% 6% of the calcium chloride is weighed, and the weighed calcium chloride is added to the above mixed solution to be stirred and dissolved to obtain a coagulation bath of the sodium alginate sponge, and the sodium alginate sponge obtained in the step B is immersed in the coagulation bath. Cross-linking curing, cross-linking curing temperature is 25 ° C ~ 40 ° C, cross-linking curing time is 15 h, after the completion of curing, the sponge is placed in the air to dry naturally to obtain calcium alginate sponge;

  4 Preparation of Calcium Alginate Sponge Dressing The calcium alginate sponge obtained in the step C was cut, packaged, and then sterilized by Co6° irradiation to obtain a calcium alginate sponge medical dressing.